sb 412515 (cat. Search Results


sb 412515 (cat. # 23249)  (Cedarlane)
90
Cedarlane sb 412515 (cat. # 23249)
HIF1αODD cleavage is reduced by cathepsins L or B inhibitors. ( a – c ). Rabbit reticulocyte lysate (RRL) was pre-incubated for 5 min at either 37 °C or 60 °C, with DMSO or inhibitor, as indicated. Recombinant HIF1αODD-GFP was added and incubated for 60 min at 37 °C. Reactions were resolved by SDS-PAGE and immunoblotted for GFP. E-64 was used at 10 μM; Q-VD-OPh, Z-VAD-FMK, calpastatin peptide, MDL-28170 calpeptin, CA-074 (CTSB inhibitor), LY3000328 (CTSS inhibitor), <t>SB</t> <t>412515</t> (CTSL inhibitor), and L-006,235 (CTSK inhibitor) were each used at 0.1, 1, and 10 μM. Blots are representative of three independent experiments. Original blots are presented in Supplementary Fig. .
Sb 412515 (Cat. # 23249), supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sb 412515 (cat. # 23249)/product/Cedarlane
Average 90 stars, based on 1 article reviews
sb 412515 (cat. # 23249) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


HIF1αODD cleavage is reduced by cathepsins L or B inhibitors. ( a – c ). Rabbit reticulocyte lysate (RRL) was pre-incubated for 5 min at either 37 °C or 60 °C, with DMSO or inhibitor, as indicated. Recombinant HIF1αODD-GFP was added and incubated for 60 min at 37 °C. Reactions were resolved by SDS-PAGE and immunoblotted for GFP. E-64 was used at 10 μM; Q-VD-OPh, Z-VAD-FMK, calpastatin peptide, MDL-28170 calpeptin, CA-074 (CTSB inhibitor), LY3000328 (CTSS inhibitor), SB 412515 (CTSL inhibitor), and L-006,235 (CTSK inhibitor) were each used at 0.1, 1, and 10 μM. Blots are representative of three independent experiments. Original blots are presented in Supplementary Fig. .

Journal: Scientific Reports

Article Title: Cathepsins L and B target HIF1α for oxygen-independent proteolytic cleavage

doi: 10.1038/s41598-024-65537-9

Figure Lengend Snippet: HIF1αODD cleavage is reduced by cathepsins L or B inhibitors. ( a – c ). Rabbit reticulocyte lysate (RRL) was pre-incubated for 5 min at either 37 °C or 60 °C, with DMSO or inhibitor, as indicated. Recombinant HIF1αODD-GFP was added and incubated for 60 min at 37 °C. Reactions were resolved by SDS-PAGE and immunoblotted for GFP. E-64 was used at 10 μM; Q-VD-OPh, Z-VAD-FMK, calpastatin peptide, MDL-28170 calpeptin, CA-074 (CTSB inhibitor), LY3000328 (CTSS inhibitor), SB 412515 (CTSL inhibitor), and L-006,235 (CTSK inhibitor) were each used at 0.1, 1, and 10 μM. Blots are representative of three independent experiments. Original blots are presented in Supplementary Fig. .

Article Snippet: Z-VAD-FMK (Cat. # ALX-260-020-M001), Q-VD-OPh (Cat. # S7311), acetyl-calpastatin 184–210 (Cat. # 2950), MDL-28170 (Cat. # S7394), calpeptin (Cat. # 0448), LY3000328 (Cat. # 29729), SB 412515 (Cat. # 23249), L-006,235 (Cat. # 28843), recombinant human cathepsin B (Cat.# 28843), and recombinant human cathepsin L (Cat.# 787504) were obtained from Cedarlane Laboratories.

Techniques: Incubation, Recombinant, SDS Page